incl. VAT plus Shipping Costs
Applyo SARS-CoV-2 RT-qPCR for quantitvative RT-qPCR analysis of RdRP, N1 and E gene of SARS-CoV-2 in FAM, TXR and Cy-5 channel.
384,00 € – 389,00 €
For in vitro use and RUO only!
Applyo Beads | |
---|---|
Shipping | Room temperature |
Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. |
Shelf life | 12 months |
Form | Lyophilized |
Concentration | Each 1x Appylo Bead contains all components for a 25 µl SARS-CoV-2 RT-qPCR-reaction |
Description | Applyo SARS-CoV-2 RT-qPCR Beads contain all reagents for detection of SARS-CoV-2 RNA in a lyophilized bead format. This includes among others a Hot-Start Taq DNA Polymerase and reverse transcriptase as well as primers and probes for SARS-CoV-2 RNA detection.
Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. |
variants | Quantity | Price | |
---|---|---|---|
12 Stripes à 8 Beads | 384,00 € | ||
96 Beads in Standard PCR Plate | 384,00 € | ||
96 Beads in Fortitude 1D PCR Plate | 386,50 € | ||
96 Beads in Fortitude 2D PCR Plate | 389,00 € |
Applyo SARS-CoV-2 RT-qPCR Beads
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the RNA target and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months.
Handling of the Applyo Beads
Open the tube containing the Applyo Bead just right before addition of RNA sample. Prepare RNA sample according to your protocol in PCR-clean water or 10 mM Tris pH 8.0-buffer. Solve the Applyo Bead directly in the tube that it is provided in.
Protocol at a glance
Component | Stock conc. | Volume in 25 µl assay | Final conc. |
---|---|---|---|
Applyo Bead | 1x | 1 µl (bead volume will add 1 µl to total reaction volume) | 1x |
RNA sample | 1 µl – 24 µl | 20 – 100,000 copies / reaction | |
Nuclease-free water | Fill up to 25 µl if required |
Recommended cycling program:
Temperature* | Time* | |
---|---|---|
50°C | 3 min. | |
95°C | 1 min. | |
95°C | 5 sec. | Repeat 45x* |
58°C | 30 sec. | |
Signal readout |
Applyo Jena GmbH
Göschwitzer Straße 22
07745 Jena
Deutschland
Phone: +49 (0)3641 554 788-0
Fax: +49 (0)3641 554 788-1
E-Mail: info@applyo-jena.com
Web: www.applyo-jena.com
Manufacturer of molecular biology reagent mixtures for research and development laboratories, as well as reagent and diagnostics manufacturers – stable at room temperature and suitable for immediate use.
Applyo Jena GmbH
Göschwitzer Straße 22
07745 Jena
Deutschland
Phone: +49 (0)3641 554 788-0
Fax: +49 (0)3641 554 788-1
E-Mail: info@applyo-jena.com
Web: www.applyo-jena.com
Manufacturer of molecular biology reagent mixtures for research and development laboratories, as well as reagent and diagnostics manufacturers – stable at room temperature and suitable for immediate use.
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