incl. VAT plus Shipping Costs
Applyo SARS-CoV-2 RT-qPCR for quantitvative RT-qPCR analysis of RdRP, N1 and E gene of SARS-CoV-2 in FAM, TXR and Cy-5 channel.
384,00 € – 389,00 €
For in vitro use and RUO only!
|Storage conditions||Store at 20°C. Short term storage at temperatures up to 30°C is also possible.|
|Shelf life||12 months|
|Concentration||Each 1x Appylo Bead contains all components for a 25 µl SARS-CoV-2 RT-qPCR-reaction|
|Description||Applyo SARS-CoV-2 RT-qPCR Beads contain all reagents for detection of SARS-CoV-2 RNA in a lyophilized bead format. This includes among others a Hot-Start Taq DNA Polymerase and reverse transcriptase as well as primers and probes for SARS-CoV-2 RNA detection.
Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well.
|12 Stripes à 8 Beads||384,00 €|
|96 Beads in Standard PCR Plate||384,00 €|
|96 Beads in Fortitude 1D PCR Plate||386,50 €|
|96 Beads in Fortitude 2D PCR Plate||389,00 €|
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the RNA target and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months.
Handling of the Applyo Beads
Open the tube containing the Applyo Bead just right before addition of RNA sample. Prepare RNA sample according to your protocol in PCR-clean water or 10 mM Tris pH 8.0-buffer. Solve the Applyo Bead directly in the tube that it is provided in.
Protocol at a glance
|Component||Stock conc.||Volume in 25 µl assay||Final conc.|
|Applyo Bead||1x||1 µl (bead volume will add 1 µl to total reaction volume)||1x|
|RNA sample||1 µl – 24 µl||20 – 100,000 copies / reaction|
|Nuclease-free water||Fill up to 25 µl if required|
Recommended cycling program:
|95°C||5 sec.||Repeat 45x*|
Applyo Jena GmbH
Manufacturer of molecular biology reagent mixtures for research and development laboratories, as well as reagent and diagnostics manufacturers – stable at room temperature and suitable for immediate use.