Applyo Jena
Applyo Jena
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RT-qPCR

RT-qPCR

RT-qPCR Liquid Master-Mix

incl. VAT plus Shipping Costs

Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler).

244,80 €

Description

For in vitro use and RUO only!

Applyo Liquid Master mix
Shipping -20°C
Storage conditions Store – 20°C. Short term storage at temperatures up to 4°C is also possible.
Shelf life 12 months
Form Liquid
Concentration 2x Master Mix
Description 2x Applyo Liquid Master Mix contains all reagents for detection of RNA. This includes among others a Hot-Start Taq DNA Polymerase and reverse transcriptase as well as all required buffer components for performing RT-qPCR reactions. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely.
Product safety

Available Options

variantsQuantityPrice 
1 Tube
244,80 €

Handling

Applyo RT-qPCR Liquid Master Mix

Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles.

Recommended RT-qPCR protocol for Applyo RT-qPCR Liquid Master Mix

Prepare RNA sample according to your protocol in PCR-clean water or 10 mM Tris pH 8.0 buffer.
Thaw Applyo Liquid Master Mix on ice upon complete thawing.

Protocol at a glance

Component Stock conc. Volume in 25 µl assay Final conc.
Applyo Liquid Master Mix 2x 12.5 µl 1x
Primer 1 µl – 12.5 µl ~ 200 – 800 nM
Probes 1 µl – 12.5 µl ~ 50 nM – 200 nM
RNA sample 1 µl – 12.5 µl 20 – 100,000 copies / reaction
Nuclease-free water Fill up to 25 µl

Recommended cycling program:

Temperature* Time*
50°C 10 min.
95°C 1 min.
95°C 10 sec. Repeat 45x*
58°C 10 sec.
72°C 20 sec.
Signal readout

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