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RT-qPCR Liquid Master-Mix

Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler).


For in vitro use and RUO only!

Applyo Liquid Master mix
Shipping -20°C
Storage conditions Store – 20°C. Short term storage at temperatures up to 4°C is also possible.
Shelf life 12 months
Form Liquid
Concentration 2x Master Mix
Description 2x Applyo Liquid Master Mix contains all reagents for detection of RNA. This includes among others a Hot-Start Taq DNA Polymerase and reverse transcriptase as well as all required buffer components for performing RT-qPCR reactions. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely.

Available Options

1 Tube


Applyo RT-qPCR Liquid Master Mix

Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles.

Recommended RT-qPCR protocol for Applyo RT-qPCR Liquid Master Mix

Prepare RNA sample according to your protocol in PCR-clean water or 10 mM Tris pH 8.0 buffer.
Thaw Applyo Liquid Master Mix on ice upon complete thawing.

Protocol at a glance

Component Stock conc. Volume in 25 µl assay Final conc.
Applyo Liquid Master Mix 2x 12.5 µl 1x
Primer 1 µl – 12.5 µl ~ 200 – 800 nM
Probes 1 µl – 12.5 µl ~ 50 nM – 200 nM
RNA sample 1 µl – 12.5 µl 20 – 100,000 copies / reaction
Nuclease-free water Fill up to 25 µl

Recommended cycling program:

Temperature* Time*
50°C 10 min.
95°C 1 min.
95°C 10 sec. Repeat 45x*
58°C 10 sec.
72°C 20 sec.
Signal readout