incl. VAT plus Shipping Costs
Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler).
268,80 € – 273,80 €
For in vitro use and RUO only!
|Storage conditions||Store at 20°C. Short term storage at temperatures up to 30°C is also possible.|
|Shelf life||12 months|
|Concentration||Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction|
|Description||Applyo RT-qPCR Beads contain all reagents for detection of RNA (and DNA) in a lyophilized bead format. This includes among others a polymerase and reverse transcriptase and buffer components for performing a RT-qPCR reaction. User only needs to add target specific primers and probes.
Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling.
|12 Stripes à 8 Beads||268,80 €|
|96 Beads in Standard PCR Plate||268,80 €|
|96 Beads in Fortitude 1D PCR Plate||271,05 €|
|96 Beads in Fortitude 2D PCR Plate||273,80 €|
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months.
Handling of Applyo Beads
Open the tube containing the Applyo Bead just right before addition of primer-probe mixture.
Solve the Applyo Bead directly in the tube that it is provided in.
Protocol at a glance
|Component||Stock conc.||Volume in 25 µl assay||Final conc.|
|Applyo Bead||1x||1 µl (bead volume will add 1 µl to total reaction volume)||1x|
|Primer||1 µl – 24 µl||~ 200 – 800 nM|
|Probes||1 µl – 24 µl||~ 50 nM – 200 nM|
|RNA sample||1 µl – 24 µl||20 – 100,000 copies / reaction|
|Nuclease-free water||Fill up to 25 µl|
Recommended cycling program for adjustment to your requirements:
|95°C||5 sec.||Repeat 45x*|