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RT-qPCR Beads for Single Reactions

Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler).

268,80 273,80 

For in vitro use and RUO only!

Applyo Beads
Shipping Room temperature
Storage conditions Store at 20°C. Short term storage at temperatures up to 30°C is also possible.
Shelf life 12 months
Form Lyophilized
Concentration Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction
Description Applyo RT-qPCR Beads contain all reagents for detection of RNA (and DNA) in a lyophilized bead format. This includes among others a polymerase and reverse transcriptase and buffer components for performing a RT-qPCR reaction. User only needs to add target specific primers and probes.

Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling.

Available Options

Cat. No.QuantityPrice 
12 Stripes à 8 Beads
96 Beads in Standard PCR Plate
96 Beads in Fortitude 1D PCR Plate
96 Beads in Fortitude 2D PCR Plate


Applyo RT-qPCR Beads

Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months.

Recommended RT-qPCR protocol for Applyo RT-qPCR Beads

Handling of Applyo Beads

Open the tube containing the Applyo Bead just right before addition of primer-probe mixture.
Solve the Applyo Bead directly in the tube that it is provided in.

Protocol at a glance

Component Stock conc. Volume in 25 µl assay Final conc.
Applyo Bead 1x 1 µl (bead volume will add 1 µl to total reaction volume) 1x
Primer 1 µl – 24 µl ~ 200 – 800 nM
Probes 1 µl – 24 µl ~ 50 nM – 200 nM
RNA sample 1 µl – 24 µl 20 – 100,000 copies / reaction
Nuclease-free water Fill up to 25 µl

Recommended cycling program for adjustment to your requirements:

Temperature* Time*
50°C 3 min.
95°C 1 min.
95°C 5 sec. Repeat 45x*
58°C 30 sec.
Signal readout