incl. VAT plus Shipping Costs
Applyo qPCR product for quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler).
144,80 €
For in vitro use RUO only!
Applyo Liquid Master mix | |
---|---|
Shipping | -20°C |
Storage conditions | Store – 20°C. Short term storage at temperatures up to 4°C is also possible. |
Shelf life | 12 months |
Form | Liquid |
Concentration | 2x Master Mix |
Description | 2x Applyo Liquid Master Mix contains all reagents for detection of DNA. This includes among others a Hot-Start Taq DNA Polymerase as well as all required buffer components for performing qPCR reactions. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Cat. No. | Quantity | Price | |
---|---|---|---|
1 Tube | 144,80 € |
Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles.
Prepare DNA sample according to your protocol in PCR-clean water or 10 mM Tris pH 8.0-buffer.
Thaw Applyo Liquid Master Mix on ice upon complete thawing.
Protocol at a glance
Component | Stock conc. | Volume in 25 µl assay | Final conc. |
---|---|---|---|
Applyo Liquid Master Mix | 2x | 12.5 µl | 1x |
Primer | 1 µl – 12.5 µl | ~ 200 – 800 nM | |
Probes | 1 µl – 12.5 µl | ~ 50 nM – 200 nM | |
DNA sample | 1 µl – 12.5 µl | 20 – 100,000 copies / reaction | |
Nuclease-free water | Fill up to 25 µl |
Recommended cycling program:
Temperature* | Time* | |
---|---|---|
95°C | 1 min. | |
95°C | 10 sec. | Repeat 45x* |
58°C | 10 sec. | |
72°C | 20 sec. | |
Signal readout |
incl. VAT
plus Shipping Costs
incl. VAT
plus Shipping Costs
Applyo Jena GmbH
Göschwitzer Straße 22
07745 Jena
Deutschland
Phone: +49 (0)3641 554 788-0
Fax: +49 (0)3641 554 788-1
E-Mail: info@applyo-jena.com
Web: www.applyo-jena.com
Manufacturer of molecular biology reagent mixtures for research and development laboratories, as well as reagent and diagnostics manufacturers – stable at room temperature and suitable for immediate use.
→ Imprint
→ Privacy Policy
→ Cookie Policy
→ Terms & Conditions
→ Cancellation policy
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Applyo Jena GmbH
Göschwitzer Straße 22
07745 Jena
Deutschland
Phone: +49 (0)3641 554 788-0
Fax: +49 (0)3641 554 788-1
E-Mail: info@applyo-jena.com
Web: www.applyo-jena.com
Manufacturer of molecular biology reagent mixtures for research and development laboratories, as well as reagent and diagnostics manufacturers – stable at room temperature and suitable for immediate use.
→ Imprint
→ Privacy Policy
→ Cookie Policy
→ Terms & Conditions
→ Cancellation policy
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