Applyo-Jena offers Applyo Beads and Liquid Mixes
Within our product portfolio we offer different Applyo Bead types for specific applications (PCR and isothermal amplification) and requirements.
For research and development in academic or industrial laboratories, we focus on features like easy and convenient application as well as save handling.
For PoC or PoN diagnostic solutions we pay close attention to long shelf life even under adverse storage conditions. Additionally, all products offer all the benefits of Applyo Beads:
Our Web-Shop is under preparation.
In the meantime please call +49 (0)3641 554 78 90 or e-mail to info@applyo-jena.com
Generic PCR Applyo products
Chose between Applyo Beads or Applyo Liquid Master Mix.
Generic Isothermal Amplification Applyo products
Chose between Applyo Beads or Applyo Liquid Master Mix.
Target specific RUO Assay Applyo products
Chose between Applyo Beads or Applyo Liquid Master Mix.
Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler).
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APGP11-96PL | 96 Beads in PCR plate | 96 | xxx.xx € | ϖ |
| APGP11-12ST | 96 Beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APGP11-100x | One 100x Bead | 100 | xxx.xx € | ϖ |
| APGP11-4x24 | 4x Beads, each 25x | 100 | xxx.xx € | ϖ |
| APGP11-96MM | 2x Liquid Master Mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo Liquid Master mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction | 2x Master Mix |
| Description | Applyo RT-qPCR Beads contain all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction in a lyophilized bead format. Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. Only Primers and Probes for user specific templates are required. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo Liquid Master Mix contains all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction as a 2x concentrated mix. Only Primers and Probes for user specific templates are required. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-qPCR Applyo Beads | RT-qPCR Applyo Liquid Master Mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection in FAM-channel (488 nm) and added dye for melting curve analysis in xxx-channel.
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APGP12-96PL | 96 Beads in PCR plate | 96 | xxx.xx € | ϖ |
| APGP12-12ST | 96 Beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APGP12-100x | One 100x Bead | 100 | xxx.xx € | ϖ |
| APGP12-4x24 | 4x Beads, each 25x | 100 | xxx.xx € | ϖ |
| APGP12-96MM | 2x Liquid Master Mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo Liquid Master Mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction | 2x Master Mix |
| Description | Applyo RT-qPCR Beads contain all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction in a lyophilized bead format. Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. Only Primers and Probes for user specific templates are required. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo Liquid Master Mix contains all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction as a 2x concentrated mix. Only Primers and Probes for user specific templates are required. Our 2x concentrated Master Mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-qPCR Applyo Beads | RT-qPCR Applyo Liquid Master Mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 3-plex (e.g. detection with 488 nm, 533 nm, 581 nm, 652 nm) wavelength depending on the filter setup of your thermocycler).
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APGP13-96PL | 96 Beads in PCR plate | 96 | xxx.xx € | ϖ |
| APGP13-12ST | 96 Beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APGP13-100x | One 100x Bead | 100 | xxx.xx € | ϖ |
| APGP13-4x24 | 4x Beads, each 25x | 100 | xxx.xx € | ϖ |
| APGP13-96MM | 2x Liquid Master Mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo Liquid Master mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction | 2x Master Mix |
| Description | Applyo RT-qPCR Beads contain all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction in a lyophilized bead format. Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. Only Primers and Probes for user specific templates are required. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo Liquid Master Mix contains all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction as a 2x concentrated mix. Only Primers and Probes for user specific templates are required. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-qPCR Applyo Beads | RT-qPCR Applyo Liquid Master Mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Handling of Applyo Beads
Open the tube containing the Applyo Bead just right before addition of primer-probe mixture.
Solve the Applyo Bead directly in the tube that it is provided in.
Protocol
Select a pair of PCR-primers and one fluorescently labeled probe per detected target.
Chose fluorescent probes according to your thermocyclers filter setup. Avoid fluorescent colors, which bleed over into different channels to avoid cross-talk between the dyes.
Component | Stock conc. | Volume in 25 µl assay | Final conc. |
Applyo Bead | 1x | 1 µl (bead volume will add 1 µl to total reaction volume) | 1x |
Primer mix | 100 µM each primer | 0.2 µl - 0.4 µl | 400 nM - 800 nM each primer |
Probe mix | 100 µM each probe | 0.05 µl - 0.1 µl | 200 nM - 400 nM each probe |
RNA sample |
| 1 µl - 21.5 µl* | 20 - 100,000 copies / reaction |
Nuclease-free water |
| Fill up to 25 µl |
|
* The amount of target, that can be added to the reaction depends on the amount and concentration of the primers and probes added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer.
Recommended cycling program:
Temperature* | Time* |
|
50°C | 1 min | |
94°C | 5 sec | |
94°C | 3 sec | Repeat 45x* |
58°C | 20 sec | |
Signal readout | ||
Select a pair of PCR-primers and one fluorescently labeled probe per detected target.
Chose fluorescent probes according to your thermocyclers filter setup. Avoid fluorescent colors, which bleed over into different channels to avoid cross-talk between the dyes.
Thaw Applyo Liquid Master Mix at room temperature and place on ice upon complete thawing.
Component | Stock conc. | Volume in 25 µl assay | Final conc. |
Applyo Liquid Master Mix | 2x | 12.5 µl | 1x |
Primer mix | 100 µM each primer | 0.2 µl - 0.4 µl | 400 nM - 800 nM each primer |
Probe mix | 100 µM each probe | 0.05 µl - 0.1 µl | 200 nM - 400 nM each probe |
RNA sample |
| 1 µl - 10 µl* | 20 - 100,000 copies / reaction |
Nuclease-free water |
| Fill up to 25 µl |
|
* The amount of target, that can be added to the reaction depends on the amount and concentration of the primers and probes added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer.
Recommended cycling program:
Temperature* | Time* |
|
50°C | 1 min | |
94°C | 5 sec | |
94°C | 3 sec | Repeat 45x* |
58°C | 20 sec | |
Signal readout | ||
Applyo qPCR product for quantitvative PCR analyses, product detection by DNA-intercalating dye in the FAM-channel (488 nm).
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APGP21-96PL | 96 Beads in PCR plate | 96 | xxx.xx € | ϖ |
| APGP21-12ST | 96 Beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APGP21-100x | One 100x Bead | 100 | xxx.xx € | ϖ |
| APGP21-4x24 | 4x Beads, each 25x | 100 | xxx.xx € | ϖ |
| APGP21-96MM | 2x Liquid Master Mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo Liquid Master Mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction | 2x Master Mix |
| Description | Applyo RT-qPCR Beads contain all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction in a lyophilized bead format. Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. Only Primers and Probes for user specific templates are required. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo Liquid Master Mix contains all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction as a 2x concentrated mix. Only primers and probes for user specific templates are required. Our 2x concentrated Master Mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-qPCR Applyo Beads | RT-qPCR Applyo Liquid Master Mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Handling of the Applyo Beads
Open the tube containing the Applyo Bead just right before addition of primer-probe mixture.
Solve the Applyo Bead directly in the tube that it is provided in.
Protocol at a glance
Component | Stock conc. | Volume in 25 µl assay | Final conc. |
Applyo Bead | 1x | 1 µl (bead volume will add 1 µl to total reaction volume) | 1x |
Primer mix | 100 µM each primer | 0.2 µl - 0.4 µl | 400 nM - 800 nM each primer |
DNA sample |
| 1 µl - 23.2 µl* | 20 - 100,000 copies / reaction |
Nuclease-free water |
| Fill up to 25 µl |
|
* The amount of target, that can be added to the reaction depends on the concentration of the primers added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare DNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer.
Recommended cycling program:
Temperature* | Time* |
|
| 94°C | 5 sec | |
94°C | 3 sec | Repeat 45x* |
58°C | 20 sec | |
Signal readout | ||
Thaw Applyo Liquid Master Mix at room temperature and place on ice upon complete thawing.
Component | Stock conc. | Volume in 25 µl assay | Final conc. |
Applyo Liquid Master Mix | 2x | 12.5 µl | 1x |
Primer mix | 100 µM each primer | 0.2 µl - 0.4 µl | 400 nM - 800 nM each primer |
DNA sample |
| 1 µl - 11.7 µl* | 20 - 100,000 copies / reaction |
Nuclease-free water |
| Fill up to 25 µl |
|
* The amount of target, that can be added to the reaction depends on the concentration of the primers added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare DNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer.
Recommended cycling program:
Temperature* | Time* |
|
| 94°C | 5 sec | |
94°C | 3 sec | Repeat 45x* |
58°C | 20 sec | |
Signal readout | ||
Applyo RT-Lmp Beads for pH-independent, reverse transcriptase – colorimetric LAMP analyses. The detection of product occurs independent of the pH of the reaction mix and is visual by a color change from red to green.
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APGI31-96PL | 96 beads in PCR plate | 96 | xxx.xx € | ϖ |
| APGI31-12ST | 96 beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APGI31-100x | One 100x bead | 100 | xxx.xx € | ϖ |
| APGI31-4x24 | 4x beads, each 25x | 100 | xxx.xx € | ϖ |
| APGI31-96MM | 2x liquid master mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo liquid master mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-LAMP-reaction | 2x master mix |
| Description | Applyo RT-LAMP Beads contain all reagents (including strand-displacement polymerase and reverse transcriptase) required for a RT-qPCR reaction in a lyophilized bead format. Each Applyo Bead is designed for a single 25 µl RT-LAMP-reaction-well. Only primers and probes for user specific templates are required. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo liquid master mix contains all reagents (including strand-displacement polymerase and reverse transcriptase) required for a RT-qPCR reaction as a 2x concentrated mix. Only primers and probes for user specific templates are required. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-LAMP Applyo Beads | RT-LAMP Applyo liquid master mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo liquid master mides are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Handling of the Beads
Open the tube containing the Applyo Bead just right before addition of primer-probe mixture.
Solve the Applyo Bead directly in the tube that it is provided in.
Protocol at a glance
Prepare 10x primer mix:
Component | Stock conc. | Volume for 100 µl 10x primer mix | Final conc. in 10x primer mix |
| Primer F1 | 100 µM | 16 µl | 16 µM |
Primer B1 | 100 µM | 16 µl | 16 µM |
Primer F3 | 100 µM | 2 µl | 2 µM |
Primer B3 | 100 µM | 2 µl | 2 µM |
Primer F2 | 100 µM | 4 µl | 4 µM |
Primer B2 | 100 µM | 4 µl | 4 µM |
Nuclease-free water | 66 µl |
|
Store primer mix at -20°C until use.
| Component | Stock conc. | Volume in 25 µl assay | Final conc. |
| Applyo LAMP Bead | 1x | 1 µl (bead volume will add 1 µl to total reaction volume | 1x |
| Primer Mix | 10x | 2.5 µl | 1x |
| RNA sample | Up to 21.5 µl* | 20 - 100,000 copies / reaction | |
| Nuclease-free water | Fill up to 25 µl |
* The amount of target, that can be added to the reaction depends on the amount and concentration of the primers and probes added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer. The sample may contain a buffer, MgCl2 in the sample is not recommended.
Resuspend Applyo Bead gently and incubate sample at 65°C for 15-30 min until color change has occured.
Do not read out the results after >35 min.
Protocol at a glance
Prepare 10x primer mix:
Component | Stock conc. | Volume for 100 µl 10x primer mix | Final conc. in 10x primer mix |
| Primer F1 | 100 µM | 16 µl | 16 µM |
Primer B1 | 100 µM | 16 µl | 16 µM |
Primer F3 | 100 µM | 2 µl | 2 µM |
Primer B3 | 100 µM | 2 µl | 2 µM |
Primer F2 | 100 µM | 4 µl | 4 µM |
Primer B2 | 100 µM | 4 µl | 4 µM |
Nuclease-free water | 66 µl |
|
Store primer mix at -20°C until use.
| Component | Stock conc. | Volume in 25 µl assay | Final conc. |
| Applyo Liquid Master Mix | 2x | 12.5 µl | 1x |
| Primer Mix | 10x | 2.5 µl | 1x |
| RNA sample | Up to 10 µl* | 20 - 100,000 copies / reaction | |
| Nuclease-free water | Fill up to 25 µl |
* The amount of target, that can be added to the reaction depends on the amount and concentration of the primers and probes added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer. The sample may contain a buffer, MgCl2 in the sample is not recommended.
Mix LAMP reaction gently and incubate sample at 65°C for 15-30 min until color change has occured.
Do not read out results after >35 min.
Applyo RT-LAMP Beads for pH-independent, reverse transcriptase – fluorescence LAMP analyses. The detection of product occurs independent of the pH of the reaction mix and is visual as fluorescent signal in the FAM channel (488 nm).
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APGI32-96PL | 96 beads in PCR plate | 96 | xxx.xx € | ϖ |
| APGI32-12ST | 96 beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APGI32-100x | One 100x bead | 100 | xxx.xx € | ϖ |
| APGI32-4x24 | 4x beads, each 25x | 100 | xxx.xx € | ϖ |
| APGI32-96MM | 2x liquid master mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo liquid master mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-LAMP-reaction | 2x master mix |
| Description | Applyo RT-LAMP Beads contain all reagents (including strand-displacement polymerase and reverse transcriptase) required for a RT-qPCR reaction in a lyophilized bead format. Each Applyo Bead is designed for a single 25 µl RT-LAMP-reaction-well. Only primers and probes for user specific templates are required. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo liquid master mix contains all reagents (including strand-displacement polymerase and reverse transcriptase) required for a RT-qPCR reaction as a 2x concentrated mix. Only primers and probes for user specific templates are required. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-LAMP Applyo Beads | RT-LAMP Applyo liquid master mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo liquid master mides are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Handling of the Beads
Open the tube containing the Applyo Bead just right before addition of primer mix.
Solve the Applyo Bead directly in the tube that it is provided in.
Protocol at a glance
Prepare 10x custom primer mix:
Component | Stock conc. | Volume for 100 µl 10x primer mix | Final conc. in 10x primer mix |
| Primer F1 | 100 µM | 16 µl | 16 µM |
Primer B1 | 100 µM | 16 µl | 16 µM |
Primer F3 | 100 µM | 2 µl | 2 µM |
Primer B3 | 100 µM | 2 µl | 2 µM |
Primer F2 | 100 µM | 4 µl | 4 µM |
Primer B2 | 100 µM | 4 µl | 4 µM |
Nuclease-free water | xx µl |
|
Store primer-probe mix at -20°C until use.
| Component | Stock conc. | Volume in 25 µl assay | Final conc. |
| Applyo LAMP Bead | 1x | 1 µl (bead volume will add 1 µl to total reaction volume | 1x |
| Primer-probe mix | 10x | 2.5 µl | 1x |
| RNA sample | Up to 21.5 µl* | 20 - 100,000 copies / reaction | |
| Nuclease-free water | Fill up to 25 µl |
* The amount of target, that can be added to the reaction depends on the amount and concentration of the primers and probes added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer. The sample may contain a buffer, MgCl2 in the sample is not recommended.
Resuspend Applyo Bead gently and incubate sample at 65°C for 15-30 min:
| Temperature | Time | |
| 65°C | 2 sec | repeat 450-900x (= 15 - 30 min) |
Protocol at a glance
Prepare 10x custom primer mix:
Component | Stock conc. | Volume for 100 µl 10x primer mix | Final conc. in 10x primer mix |
| Primer F1 | 100 µM | 16 µl | 16 µM |
Primer B1 | 100 µM | 16 µl | 16 µM |
Primer F3 | 100 µM | 2 µl | 2 µM |
Primer B3 | 100 µM | 2 µl | 2 µM |
Primer F2 | 100 µM | 4 µl | 4 µM |
Primer B2 | 100 µM | 4 µl | 4 µM |
Fluorescently labelled probe | 100 µM |
|
|
Nuclease-free water | xx µl |
|
Store primer-probe mix at -20°C until use.
| Component | Stock conc. | Volume in 25 µl assay | Final conc. |
| Applyo Liquid Master Mix | 2x | 12.5 µl | 1x |
| Primer-probe mix | 10x | 2.5 µl | 1x |
| RNA sample | Up to 10 µl* | 20 - 100,000 copies / reaction | |
| Nuclease-free water | Fill up to 25 µl |
* The amount of target, that can be added to the reaction depends on the amount and concentration of the primers and probes added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer. The sample may contain a buffer, MgCl2 in the sample is not recommended.
Mix Applyo reaction gently and incubate sample at 65°C for 15-30 min:
| Temperature | Time | |
| 65°C | 2 sec | repeat 450-900x (= 15 - 30 min) |
Applyo RT-Lmp Beads for pH-independent, reverse transcriptase – fluorescence LAMP analyses. The detection of product occurs independent of the pH of the reaction mix and is visual as colorimetric signal (red - green color shift) and fluorescent signal in the FAM channel (488 nm).
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APGI32-96PL | 96 beads in PCR plate | 96 | xxx.xx € | ϖ |
| APGI32-12ST | 96 beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APGI32-100x | One 100x bead | 100 | xxx.xx € | ϖ |
| APGI32-4x24 | 4x beads, each 25x | 100 | xxx.xx € | ϖ |
| APGI32-96MM | 2x liquid master mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo liquid master mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-LAMP-reaction | 2x master mix |
| Description | Applyo RT-LAMP Beads contain all reagents (including strand-displacement polymerase and reverse transcriptase) required for a RT-qPCR reaction in a lyophilized bead format. Each Applyo Bead is designed for a single 25 µl RT-LAMP-reaction-well. Only primers and probes for user specific templates are required. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo liquid master mix contains all reagents (including strand-displacement polymerase and reverse transcriptase) required for a RT-qPCR reaction as a 2x concentrated mix. Only primers and probes for user specific templates are required. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-LAMP Applyo Beads | RT-LAMP Applyo liquid master mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo liquid master mides are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Handling of the Beads
Open the tube containing the Applyo Bead just right before addition of primer mix.
Solve the Applyo Bead directly in the tube that it is provided in.
Protocol at a glance
Prepare 10x custom primer mix:
Component | Stock conc. | Volume for 100 µl 10x primer mix | Final conc. in 10x primer mix |
| Primer F1 | 100 µM | 16 µl | 16 µM |
Primer B1 | 100 µM | 16 µl | 16 µM |
Primer F3 | 100 µM | 2 µl | 2 µM |
Primer B3 | 100 µM | 2 µl | 2 µM |
Primer F2 | 100 µM | 4 µl | 4 µM |
Primer B2 | 100 µM | 4 µl | 4 µM |
Nuclease-free water | xx µl |
|
Store primer-probe mix at -20°C until use.
| Component | Stock conc. | Volume in 25 µl assay | Final conc. |
| Applyo LAMP Bead | 1x | 1 µl (bead volume will add 1 µl to total reaction volume | 1x |
| Primer-probe mix | 10x | 2.5 µl | 1x |
| RNA sample | Up to 21.5 µl* | 20 - 100,000 copies / reaction | |
| Nuclease-free water | Fill up to 25 µl |
* The amount of target, that can be added to the reaction depends on the amount and concentration of the primers and probes added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer. The sample may contain a buffer, MgCl2 in the sample is not recommended.
Resuspend Applyo Bead gently and incubate sample at 65°C for 15-30 min.
The detection of product occurs independent of the pH of the reaction mix and is visual as color change from red - green and fluorescent signal in the FAM channel (488 nm):
| Temperature | Time | |
| 65°C | 2 sec | repeat 450-900x (= 15 - 30 min) |
Protocol at a glance
Prepare 10x custom primer mix:
Component | Stock conc. | Volume for 100 µl 10x primer mix | Final conc. in 10x primer mix |
| Primer F1 | 100 µM | 16 µl | 16 µM |
Primer B1 | 100 µM | 16 µl | 16 µM |
Primer F3 | 100 µM | 2 µl | 2 µM |
Primer B3 | 100 µM | 2 µl | 2 µM |
Primer F2 | 100 µM | 4 µl | 4 µM |
Primer B2 | 100 µM | 4 µl | 4 µM |
Fluorescently labelled probe | 100 µM |
|
|
Nuclease-free water | xx µl |
|
Store primer-probe mix at -20°C until use.
| Component | Stock conc. | Volume in 25 µl assay | Final conc. |
| Applyo Liquid Master Mix | 2x | 12.5 µl | 1x |
| Primer-probe mix | 10x | 2.5 µl | 1x |
| RNA sample | Up to 10 µl* | 20 - 100,000 copies / reaction | |
| Nuclease-free water | Fill up to 25 µl |
* The amount of target, that can be added to the reaction depends on the amount and concentration of the primers and probes added to the reaction. The total reaction volume of 25 µl should not be exceeded.
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer. The sample may contain a buffer, MgCl2 in the sample is not recommended.
Mix Applyo reaction gently and incubate sample at 65°C for 15-30 min.
The detection of product occurs independent of the pH of the reaction mix and is visual as color change from red - green and fluorescent signal in the FAM channel (488 nm):
| Temperature | Time | |
| 65°C | 2 sec | repeat 450-900x (= 15 - 30 min) |
Applyo RT-qPCR product for detection of SARS-CoV-2 RNA via reverse transcriptase – quantitvative PCR. Probe mediated detection occurs in the FAM-channel (488 nm).
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APTS11-96PL | 96 Beads in PCR plate | 96 | xxx.xx € | ϖ |
| APTSP11-12ST | 96 Beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APTS11-100x | One 100x Bead | 100 | xxx.xx € | ϖ |
| APTS11-4x24 | 4x Beads, each 25x | 100 | xxx.xx € | ϖ |
| APTS11-96MM | 2x Liquid Master Mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo Liquid Master mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction | 2x Master Mix |
| Description | Applyo RT-qPCR Beads contain all reagents for detection of SARS-CoV-2 RNA in a lyophilized bead format. This includes among others a polymerase and reverse transcriptase as well as primers and probes for a RT-qPCR reaction. Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo Liquid Master Mix contains all reagents for detection of SARS-CoV-2 RNA as a 2x concentrated master mix. This includes among others a polymerase and reverse transcriptase as well as primers and probes for a RT-qPCR reaction. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-qPCR Applyo Beads | RT-qPCR Applyo Liquid Master Mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Handling of the Applyo Beads
Open the tube containing the Applyo Bead just right before addition of primer-probe mixture.
Solve the Applyo Bead directly in the tube that it is provided in.
Protocol at a glance
Component | Stock conc. | Volume in 25 µl assay | Final conc. |
Applyo Bead | 1x | 1 µl (bead volume will add 1 µl to total reaction volume) | 1x |
RNA sample |
| 1 µl - 24 µl | 20 - 100,000 copies / reaction |
Nuclease-free water |
| Fill up to 25 µl |
|
Prepare RNA sample in PCR-clean water or 10 mM Tris pH 8.0-buffer.
Recommended cycling program:
Temperature* | Time* |
|
50°C | 1 min | |
94°C | 5 sec | |
94°C | 3 sec | Repeat 45x* |
58°C | 20 sec | |
Signal readout | ||
Prepare RNA sample according to your protocol in PCR-clean water or 10 mM Tris pH 8.0-buffer.
Thaw Applyo Liquid Master Mix at room temperature and place on ice upon complete thawing.
Component | Stock conc. | Volume in 25 µl assay | Final conc. |
Applyo Liquid Master Mix | 2x | 12.5 µl | 1x |
RNA sample |
| 1 µl - 12.5 µl | 20 - 100,000 copies / reaction |
Nuclease-free water |
| Fill up to 25 µl |
|
Recommended cycling program:
Temperature* | Time* |
|
50°C | 1 min | |
94°C | 5 sec | |
94°C | 3 sec | Repeat 45x* |
58°C | 20 sec | |
Signal readout | ||
For research and development in academic or industrial laboratories, we focus on features like easy and convenient application as well as save handling.
For PoC or PoN diagnostic solutions we pay close attention to long shelf life even under adverse storage conditions. Additionally, all products offer all the benefits of Applyo Beads:
Applyo-Jena offers Applyo Beads and liquid mixes
Generic PCR Applyo products
Chose between Applyo Beads or Applyo Liquid Master Mix.
Generic Isothermal Amplification Applyo products
Chose between Applyo Beads or Applyo Liquid Master Mix.
Target specific RUO Assay Applyo products
Chose between Applyo Beads or Applyo Liquid Master Mix.
Applyo RT-qPCR product for reverse transcriptase – quantitvative PCR analyses with target detection possible in 5-plex (e.g. detection with 488 nm, 533 nm, 552 nm, 581 nm, 652 nm, 678 nm wave length depending on the filter setup of your thermocycler).
Chose between Applyo Beads and Liquid Master Mix.
| Cat. No. | Description | No. of Assays | Price (€) | Buy |
| APGP11-96PL | 96 Beads in PCR plate | 96 | xxx.xx € | ϖ |
| APGP11-12ST | 96 Beads in 12x8er PCR stripes | 96 | xxx.xx € | ϖ |
| APGP11-100x | One 100x Bead | 100 | xxx.xx € | ϖ |
| APGP11-4x24 | 4x Beads, each 25x | 100 | xxx.xx € | ϖ |
| APGP11-96MM | 2x Liquid Master Mix for 96 rcts. | 96 | xxx.xx € | ϖ |
For in vitro use only!
| Applyo Beads | Applyo Liquid Master mix | |
| Shipping | Room temperature | -20°C |
| Storage conditions | Store at 20°C. Short term storage at temperatures up to 30°C is also possible. | Store - 20°C. Short term storage at temperatures up to 4°C is also possible. |
| Shelf life | 12 months | 12 months |
| Form | Lyophilized | Liquid |
| Concentration | Each 1x Appylo Bead contains all components for a 25 µl RT-qPCR-reaction | 2x Master Mix |
| Description | Applyo RT-qPCR Beads contain all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction in a lyophilized bead format. Each Applyo Bead is designed for a single 25 µl RT-qPCR-reaction-well. Only Primers and Probes for user specific templates are required. The beads are pre-mixed, guaranteeing reliable amplification results with minimal pipetting effort. Our ready-to-use bead-format with instantly resuspending reaction mixes ensures time-saving fast experimental setup with low risk of cross-contamination during handling. The different product formats (25x and 100x Beads as a well as Liquid Maser Mix) allow individual adjustment to different PCR-volumes used. | 2x Applyo Liquid Master Mix contains all reagents (including polymerase and reverse transcriptase) required for a RT-qPCR reaction as a 2x concentrated mix. Only Primers and Probes for user specific templates are required. Our 2x concentrated master mix format offers a quick experimental setup and minimizes well-to-well and experiment-experiment variations substantially. The reaction volume can be chosen freely. |
Handling
| RT-qPCR Applyo Beads | RT-qPCR Applyo Liquid Master Mix |
Applyo Beads are stable under ambient conditions. Exposure to high moisture (>80 % rH) for more than 30 minutes or placing beads on ice may result in moisture condensation and may impair the PCR-performance due to the reduced solubility of the Applyo Beads. Therefore, limit the exposure to humidity (e.g. in the air) to a minimum until the bead is reconstituted with the primer-probe mixture and carefully reseal the package with remaining Applyo Beads if only a part of the beads is applied. Resealed Applyo Beads should be used within 3 months. | Applyo Liquid Master Mixes are stable at -20°C, short time storage at 4°C is also possible. Avoid extended freeze-thaw cycles. |
Applyo Jena GmbH
Hans-Knöll-Straße 6
07745 Jena
Deutschland
Phone: +49 (0)3641 554 788-0
Fax: +49 (0)3641 554 788-1
E-Mail: info@applyo-jena.com
Web: www.applyo-jena.com
Manufacturer of molecular biology reagent mixtures for research and development laboratories, as well as reagent and diagnostics manufacturers – stable at room temperature and suitable for immediate use.
Applyo Jena GmbH
Hans-Knöll-Straße 6
07745 Jena
Deutschland
Phone: +49 (0)3641 554 788-0
Fax: +49 (0)3641 554 788-1
E-Mail: info@applyo-jena.com
Web: www.applyo-jena.com
Manufacturer of molecular biology reagent mixtures for research and development laboratories, as well as reagent and diagnostics manufacturers – stable at room temperature and suitable for immediate use.
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